Before preparing libraries, record information about your samples using the available options for your sequencing system.
Use this table to help determine your SureCell loading concentration. Loading concentrations presented in this table are based on BioAnalyzer quantification. If you are quantifying with another method, you may need to optimize the loading concentration.
| System | Document | Loading Concentration |
|---|---|---|
| NextSeq | NextSeq System Custom Primers Guide (document # 15057456) | 2.7-3 pM |
| HiSeq 2500 | HiSeq System Custom Primers Guide (document # 15061846) | 18-20 pM |
| HiSeq 3000/4000 | HiSeq System Custom Primers Guide (document # 15061846) | 350-400 pM |
| MiSeq | MiSeq System Custom Primers Guide (document # 15041638) | 25-32 pM |
HiSeq System Custom Primers Guide (document # 15061846) |