Choose from the following options to record information about your samples, such as sample ID and which index adapters are used for pooling.
See MiSeq Applications and Methods for possible applications.
After preparing libraries, denature and dilute libraries to a loading concentration of 6–20 pM. For instructions, see the MiSeq System Denature and Dilute Libraries Guide.
Use the compatible version of the MiSeq Control Software (MCS) for your flow cell. For more information on flow cells, see MiSeq Reagent Kit.
Flow Cell |
Cap Color |
Compatible MCS Version |
Standard Flow Cell in MiSeq Reagent Kit v3 (19 tiles) |
Clear |
MCS v2.3, or later |
PGS Flow Cell in MiSeq Reagent Kit v3 (19 tiles) |
Clear |
MCS v2.4, or later |
Standard Flow Cell in MiSeq Reagent Kit v2 (14 tiles) |
Clear |
MCS v2.0, or later |
Micro Flow Cell in MiSeq Reagent Micro Kit v2 (4 tiles) |
Green |
MCS v2.1, or later |
Nano Flow Cell in MiSeq Reagent Nano Kit v2 (2 tiles) |
Yellow |
MCS v2.0, or later |