NextSeq 500 Compatible Products
NextSeq 500 System
Compatible Products
Sample Information
Choose from the following options to record information about your samples, such as sample ID and which index adapters are used for pooling.
Library Preparation
See NextSeq Applications and Methods for possible applications.
After library prep, denature and dilute libraries to a loading concentration of ~1.8 pM. For instructions, see NextSeq System Denature and Dilute Libraries Guide.
Run Components
Use the compatible version of the NextSeq Control Software (NCS) for your kit. For kit details, see NextSeq 500 Reagent Kit.
Kit Version |
NCS Version |
NextSeq 500/550 v2.5 Kit |
NCS v2.2.0 |
NextSeq 500/550 v2 Kit |
NCS v1.4, or later |
NextSeq 500/550 Kit (v1) |
NCS v1.3, or earlier |
TG NextSeq 500 High Output Kit |
NCS v1.3 |
Data Analysis
Use Sequencing Analysis Viewer or BaseSpace Sequence Hub to monitor real-time quality metrics during a run.
To analyze sequencing data, choose from the following options.
- Local Run Manager
- BaseSpace Sequence Hub
- bcl2fastq2 Conversion Software—Convert sequencing output for third-party analysis solutions when sequencing in Manual mode.