Requirements & Compatibility

Sample Information

Choose from the following options to record information about your samples, such as sample ID and which index adapters are used for pooling.

• Local Run Manager
• BaseSpace Sequence Hub
• Illumina Experiment Manager (Manual mode)

Library Preparation

See NextSeq Applications and Methods for possible applications.

After library prep, denature and dilute libraries to a loading concentration of ~1.8 pM. For instructions, see NextSeq System Denature and Dilute Libraries Guide.

BeadChip Preparation

The system can scan BeadChips prepared using the following kits.

• Infinium MethylationEPIC BeadChip Kit
• CytoSNP-850K BeadChip
• HumanCytoSNP-12 BeadChip
• HumanKaryomap-12 BeadChip

Run Components

Use the compatible version of the NextSeq Control Software (NCS) for your kit. For kit details, see NextSeq 500 Reagent Kit.

Data Analysis

Sequencing

Use Sequencing Analysis Viewer or BaseSpace Sequence Hub to monitor real-time quality metrics during a sequencing run.

To analyze sequencing data, choose from the following options.

• Local Run Manager
• BaseSpace Sequence Hub
• bcl2fastq2 Conversion Software—Convert sequencing output for third-party analysis solutions when sequencing in Manual mode.

Scanning

Use BlueFuse Multi software to analyze data from BeadChips.