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TruSight Oncology 500 Contents & Storage

 

Box 1 (Pre-Amp) Library Prep

DNA/RNA bundle customers receive one box. DNA kit customers do not receive this box.

Quantity Reagent Description Storage
1 FSM First Strand Synthesis Mix  -25°C to -15°C
1 SSM Second Strand Mix -25°C to -15°C
1 EPH Elute, Prime, Fragment High Mix 3 -25°C to -15°C
1 RVT Reverse Transcriptase -25°C to -15°C
Box 2 (Pre-Amp) Library Prep
Quantity Reagent Description Storage
2 UMI1 UMI Adapters v1 -25°C to -15°C
Box 3 (Pre-Amp) Library Prep
Quantity Reagent Description Storage
2 ERA1-A End Repair A-tailing Enzyme Mix 1 -25°C to -15°C
2 ERA1-B End Repair A-tailing Buffer 1 -25°C to -15°C
2 ALB1 Adapter Ligation Buffer 1 -25°C to -15°C
2 LIG3 DNA Ligase 3 -25°C to -15°C
2 SUA1 Short Universal Adapters 1 -25°C to -15°C
2 STL Stop Ligation Buffer -25°C to -15°C
2 EPM Enhanced PCR Mix -25°C to -15°C
Box 4 (Pre-Amp) Library Prep
Quantity Reagent Description Storage
1 RSB Resuspension Buffer 2°C to 8°C or -25°C to -15°C
2 SPB Sample Purification 2°C to 8°C
1 TEB TE Buffer 2°C to 8°C
Box 5 (Pre-Amp) Library Prep Unique PCR Index Primer Mixes 
Quantity Reagent Description Storage
1 UP01 Unique Index Mix Primer 01 -25°C to -15°C
1 UP02 Unique Index Mix Primer 02 -25°C to -15°C
1 UP03 Unique Index Mix Primer 03 -25°C to -15°C
1 UP04 Unique Index Mix Primer 04 -25°C to -15°C
1 UP05 Unique Index Mix Primer 05 -25°C to -15°C
1 UP06 Unique Index Mix Primer 06 -25°C to -15°C
1 UP07 Unique Index Mix Primer 07 -25°C to -15°C
1 UP08 Unique Index Mix Primer 08 -25°C to -15°C
1 UP09 Unique Index Mix Primer 09 -25°C to -15°C
1 UP10 Unique Index Mix Primer 10 -25°C to -15°C
1 UP11 Unique Index Mix Primer 11 -25°C to -15°C
1 UP12 Unique Index Mix Primer 12 -25°C to -15°C
1 UP13 Unique Index Mix Primer 13 -25°C to -15°C
1 UP14 Unique Index Mix Primer 14 -25°C to -15°C
1 UP15 Unique Index Mix Primer 15 -25°C to -15°C
1 UP16 Unique Index Mix Primer 16 -25°C to -15°C
Box 6 (Post-Amp) Enrichment
Quantity Reagent Description Storage
2 TCB1 Target Capture Buffer 1 2°C to 8°C
2 SMB Streptavidin Magnetic Beads 2°C to 8°C
2 HP3 2N NaOH 2°C to 8°C
2 ET2 Elution Target 2 2°C to 8°C
2 LNB1 Library Normalization Beads 1 2°C to 8°C
2 LNW1 Library Normalization Wash 1 2°C to 8°C
1 RSB Resuspension Buffer 2°C to 8°C or -25°C to -15°C
2 SPB Sample Purification Beads 2°C to 8°C
2 LNS1 Library Normalization Storage 1 15°C to 30°C
Box 7 (Post-Amp) Enrichment
Quantity Reagent Description Storage
2 TCA1 Target Capture Additive 1 -25°C to -15°C
1 EEW2 Enhanced Enrichment Wash 2 -25°C to -15°C
3 EE2 Enrichment Elution 2 -25°C to -15°C
2 EPM Enhanced PCR Mix -25°C to -15°C
2 PPC3 PCR Primer Cocktail 3 -25°C to -15°C
1 LNA1 Library Normalization Additives 1 -25°C to -15°C
Box 8 (Post-Amp) Enrichment

DNA/RNA bundle customers receive one box. DNA kit customers receive two of this box.

Quantity Reagent Description Storage
1 OPD2 Oncology DNA Probes Master Pool 2  -25°C to -15°C
Box 9 TruSight Oncology 500 Kit Content Set

DNA/RNA bundle customers receive one box. DNA kit customers do not receive this box.

Quantity Reagent Description Storage
1 OPR1 Oncology RNA Probes Master Pool  -25°C to -15°C
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