Illumina DNA PCR-Free Libraries require the use of custom primers. Please see the Illumina DNA PCR-Free Prep product page for ordering information. Use of associated library prep kit definition files still requires manual selection of custom primers in run setup.
When using NovaSeq v1.0 reagents, follow the NovaSeq Series Custom Primers Guide for information on using the custom sequencing primer Read 1 (VP10).
When using NovaSeq v1.5 (or newer) reagents, no custom primer kit is required. Cartridges in the NovaSeq v1.5 reagent kit contain the custom sequencing primer.
This kit is compatible with IDT for Illumina DNA/RNA UD Indexes.