Before preparing libraries, record information about your samples using an option available for the NextSeq 550 System.
The AmpliSeq for Illumina Transcriptome Human Gene Expression Panel is a highly multiplexed targeted resequencing assay for measuring expression levels of over 20,000 human RefSeq genes in one assay.
| Number of Pools | Concentration | Number of Amplicons | Average Amplicon Length (bp) | Average Library Length (bp) |
|---|---|---|---|---|
| 1 | 2.5X | 20,802 | 104 | 244 |
Completing the AmpliSeq for Illumina Transcriptome Human Gene Expression Panel requires the panel and the following library prep, cDNA synthesis, and index kits. The AmpliSeq for Illumina Library PLUS Kit is available in 24-, 96-, and 384-reaction configurations.
For each RNA sample, the AmpliSeq for Illumina Transcriptome Human Gene Expression Panel generates one RNA library. A sample is a specimen of nucleic acid from one source, while a library is nucleic acids from a sample after preparation. A library has the ends required for sequencing.
Preparing the appropriate number of samples requires the following number and combination of kits.
| Number of Samples | 24 Samples | 96 Samples | 384 Samples |
|---|---|---|---|
| Number of Libraries | 24 Libraries | 96 Libraries | 384 Libraries |
| AmpliSeq for Illumina Transcriptome Human Gene Expression Panel | 1 | 4 | 16 |
| AmpliSeq Library PLUS For Illumina | 1 24-reaction kit | 1 96-reaction kit | 1 384-reaction kit |
| AmpliSeq CD Set A (96 reaction, 96 indexes) | 1 | 1 | 4 |
| cDNA Synthesis | 1 | 1 | 2 |
Sequence AmpliSeq for Illumina Transcriptome Human Gene Expression Panel libraries using the following systems and reagent kits.
| System | Reagent Kit | Samples per Run |
|---|---|---|
| MiniSeq System | MiniSeq High Output Reagent Kit | 3 |
| MiSeq System | MiSeq Reagent Kit v3 | 3 |
| NextSeq System | NextSeq Mid Output v2 Kit | 12 |
| NextSeq High Output v2 Kit | 40 |