Before preparing libraries, record information about your samples using an option available for your sequencing system:
AmpliSeq for Illumina TCR beta-SR Panel is a highly multiplexed targeted resequencing panel intended for use with multiple sample types, including formalin-fixed paraffin-embedded (FFPE) tumor samples.
| Component | Number of Pools | Concentration |
|---|---|---|
| DNA and RNA | 1 | 5X |
Completing the AmpliSeq for Illumina TCR beta-SR protocol requires the panel and the following library prep kit and index kit.
For each DNA and RNA sample, the AmpliSeq for Illumina TCR beta-SR Panel generates one DNA and one RNA library. A sample is a specimen of nucleic acid from one source, while a library is nucleic acids from a sample after preparation. Samples can be DNA, RNA, or paired. A library has the ends required for sequencing.
Preparing the appropriate number of samples requires the following number and combination of kits.
| Number of Samples | 24 Samples | 96 Samples | 384 Samples |
|---|---|---|---|
| Number of Libraries | 24 Libraries | 96 Libraries | 384 Libraries |
| AmpliSeq for Illumina TCR beta-SR Panel | 1 | 5 | 16 |
| AmpliSeq Library PLUS For Illumina | 1 24-reaction kit (20019101) | 1 96-reaction kit (20019102) | 1 384-reaction kit (20019103) |
| AmpliSeq CD Set A (96 reaction, 96 indexes) | 1 | 1 | 4 |
| AmpliSeq UD Indexes for Illumina (24 Indexes, 24 Samples) | 1 | 4 | 16 |
| [RNA] cDNA Synthesis | 1 | 1 | 4 |
Sequence AmpliSeq for Illumina TCR beta-SR Panel libraries using the following systems and reagent kits.
| System | Reagent Kit |
|---|---|
| MiniSeq System | MiniSeq Mid Output Reagent Kit |
| MiniSeq High Output Reagent Kit | |
| NextSeq System | NextSeq Mid Output v2 Kit |
| NextSeq High Output v2 Kit |