Some dual-indexed libraries require sequencing primers in the TruSeq Dual Index Sequencing Primer Box. These primers replace primers provided in the reagent kits:
For information on compatible libraries, see HiSeq 2000 Requirements & Compatibility. For clustering and sequencing instructions, see the cBot 2 System Guide or cBot System Guide and the HiSeq 2000 System Guide.
Reagent Name |
Storage |
Description |
HP9 |
-25° to -15°C |
Index 2 (i5) Sequencing Primer Mix |
HP10 |
-25° to -15°C |
Read 1 Sequencing Primer Mix |
HP12 |
-25° to -15°C |
Index 1 (i7) Sequencing Primer Mix |
Reagent Name |
Storage |
Description |
HP10 |
-25° to -15°C |
Read 1 Sequencing Primer Mix |
HP11 |
-25° to -15°C |
Read 2 Sequencing Primer Mix |
HP12 |
-25° to -15°C |
Index 1 (i7) Sequencing Primer Mix |