Cluster the flow cell on the cBot 2 System or cBot System before sequencing on the HiSeq 2000 System.
Sequencing primers provided in the TruSeq Dual Index Sequencing Primer Box are required for sequencing the following libraries on the HiSeq 2000:
For more information, see HiSeq 2000 Primers.
Use the compatible version of HiSeq Control Software (HCS) and Real-Time Analysis (RTA) software for your kit. For kit details, see HiSeq 2000 Reagent Kits.
Cluster Kit | SBS Kit | HiSeq Software |
HiSeq Cluster Kit v4 | HiSeq SBS Kit v4 | HCS v2.2/RTA v1.18, or later |
TruSeq Cluster Kit v3 | TruSeq SBS Kit v3 | HCS v1.4/RTA v1.12, or later |
Kit | cBot Software Version | HiSeq Software |
TruSeq Dual Index Sequencing Primer Box | cBot Software v1.4.36, or later cBot Recipe Installer v1.0.13 |
HCS v1.5/RTA v1.13, or later |