Quantity |
Reagent |
Description |
1 |
HP3 |
Denaturation Solution |
1 |
HT2 |
Wash Buffer |
1 |
RMR |
Resynthesis Mix |
1 |
AT2 |
100% Formamide |
1 |
APM2 |
Amplification Premix |
1 |
AMX2 |
Amplification Mix 2 |
1 |
LMX2 |
Linearization Mix 2 |
1 |
BMX |
Blocking Mix |
1 |
HP7* |
Read 2 Sequencing Primer Mix |
* Instead of HP7, Nextera libraries require HP11 provided in the TruSeq Dual Index Sequencing Primer Box.
Quantity |
Kit Component |
Description |
1 |
cBot Reagent Plate |
cBot PE reagent plate |
1 |
HT1 |
Hybridization Buffer |
Quantity |
Reagent |
Description |
1 |
cBot Reagent Plate |
cBot SR reagent plate |
1 |
HT1 |
Hybridization Buffer |
Quantity |
Reagent |
Description |
1 |
HP3 |
Denaturation Solution |
1 |
HT2 |
Wash Buffer |
1 |
HP8* |
Index Sequencing Primer |
* Instead of HP8, Nextera libraries require HP12 provided in the TruSeq Dual Index Sequencing Primer Box.
Quantity |
Component |
Purpose |
4 |
Flow cell gaskets |
Replace the flow cell gaskets between runs. |
8 |
Funnel bottle caps |
Replace SBS reagent bottle caps before loading reagents. |
1 |
ICB instrument tube |
Divide ICB for Read 2. |
Reagents are labeled with a 3-letter acronym plus a numeric designator that indicates the kit size
Quantity |
Reagent |
Description |
Storage Temperature |
1 |
PW1 |
Wash Buffer |
-25°C to 30°C |
1 |
ICB |
Incorporation Mix Buffer |
2°C to 8°C |
1 |
SB1 |
High Salt Buffer |
2°C to 8°C |
2 |
SB2 |
Incorporation Wash Buffer |
2°C to 8°C |
1 |
SB3 |
Cleavage Buffer |
2°C to 8°C |
Quantity |
Reagent |
Description |
1 |
CMR |
Cleavage Mix Reagent |
1 |
SRE |
Enhanced Scan Mix Reagent |
1 |
EDP |
Enhanced DNA Polymerase |
4 |
LFN36 |
Long Read Nucleotide Mix |
Quantity |
Reagent |
Description |
Storage Temperature |
1 |
PW1 |
Wash Buffer |
-25°C to 30°C |
1 |
ICB |
Incorporation Mix Buffer |
2°C to 8°C |
1 |
SB1 |
High Salt Buffer |
2°C to 8°C |
2 |
SB2 |
Incorporation Buffer |
2°C to 8°C |
1 |
SB3 |
Cleavage Buffer |
2°C to 8°C |
Quantity |
Reagent |
Description |
1 |
CMR |
Cleavage Mix Reagent |
1 |
SRE |
Enhanced Scanning Mix Reagent |
1 |
EDP |
Enhanced DNA Polymerase |
1 |
LFN36 |
Long Read Nucleotide Mix |
See Safety Data Sheets.