| Quantity | Reagent | Description | 
| 1 | HP3 | Denaturation Solution | 
| 1 | HT2 | Wash Buffer | 
| 1 | RMR | Resynthesis Mix | 
| 1 | AT2 | 100% Formamide | 
| 1 | APM2 | Amplification Premix | 
| 1 | AMX2 | Amplification Mix 2 | 
| 1 | LMX2 | Linearization Mix 2 | 
| 1 | BMX | Blocking Mix | 
| 1 | HP7* | Read 2 Sequencing Primer Mix | 
* Instead of HP7, Nextera libraries require HP11 provided in the TruSeq Dual Index Sequencing Primer Box.
| Quantity | Kit Component | Description | 
| 1 | cBot Reagent Plate | cBot PE reagent plate | 
| 1 | HT1 | Hybridization Buffer | 
| Quantity | Reagent | Description | 
| 1 | cBot Reagent Plate | cBot SR reagent plate | 
| 1 | HT1 | Hybridization Buffer | 
| Quantity | Reagent | Description | 
| 1 | HP3 | Denaturation Solution | 
| 1 | HT2 | Wash Buffer | 
| 1 | HP8* | Index Sequencing Primer | 
* Instead of HP8, Nextera libraries require HP12 provided in the TruSeq Dual Index Sequencing Primer Box.
| Quantity | Component | Purpose | 
| 4 | Flow cell gaskets | Replace the flow cell gaskets between runs. | 
| 8 | Funnel bottle caps | Replace SBS reagent bottle caps before loading reagents. | 
| 1 | ICB instrument tube | Divide ICB for Read 2. | 
Reagents are labeled with a 3-letter acronym plus a numeric designator that indicates the kit size
| Quantity | Reagent | Description | Storage Temperature | 
| 1 | PW1 | Wash Buffer | -25°C to 30°C | 
| 1 | ICB | Incorporation Mix Buffer | 2°C to 8°C | 
| 1 | SB1 | High Salt Buffer | 2°C to 8°C | 
| 2 | SB2 | Incorporation Wash Buffer | 2°C to 8°C | 
| 1 | SB3 | Cleavage Buffer | 2°C to 8°C | 
| Quantity | Reagent | Description | 
| 1 | CMR | Cleavage Mix Reagent | 
| 1 | SRE | Enhanced Scan Mix Reagent | 
| 1 | EDP | Enhanced DNA Polymerase | 
| 4 | LFN36 | Long Read Nucleotide Mix | 
| Quantity | Reagent | Description | Storage Temperature | 
| 1 | PW1 | Wash Buffer | -25°C to 30°C | 
| 1 | ICB | Incorporation Mix Buffer | 2°C to 8°C | 
| 1 | SB1 | High Salt Buffer | 2°C to 8°C | 
| 2 | SB2 | Incorporation Buffer | 2°C to 8°C | 
| 1 | SB3 | Cleavage Buffer | 2°C to 8°C | 
| Quantity | Reagent | Description | 
| 1 | CMR | Cleavage Mix Reagent | 
| 1 | SRE | Enhanced Scanning Mix Reagent | 
| 1 | EDP | Enhanced DNA Polymerase | 
| 1 | LFN36 | Long Read Nucleotide Mix | 
See Safety Data Sheets.