Cluster the flow cell on the cBot 2 System or cBot System before sequencing on the HiSeq 1000 System.
Sequencing primers provided in the TruSeq Dual Index Sequencing Primer Box are required for sequencing the following libraries on the HiSeq 1000:
Use the compatible version of HiSeq Control Software (HCS) and Real-Time Analysis (RTA) software for your kit. For kit details, see HiSeq 1000 Reagent Kits.
Kit Name | cBot Software | HiSeq Control Software |
---|---|---|
TruSeq Cluster Kit v3 | cBot v1.4, or later | HCS v1.4, or later/RTA v1.12, or later |
TruSeq SBS Kit v3 | Not applicable | HCS v1.4, or later/RTA v1.12, or later |
TruSeq Dual Index Sequencing Primer Box | cBot Software v1.4.36, or later cBot Recipe Installer v1.0.13 |
HCS v1.5/RTA v1.13. or later |