Contents & Storage

The TruSight RNA Fusion Panel (48 samples) is available in a Set A and a Set B. When used together, sets A and B allow for pooling up to 24 samples using the 12 different indexes in each kit.

Box 1, Store at -25°C to -15°C

Quantity

Reagent

Description

1

PMM

PCR Master Mix

1

PPC

PCR Primer Cockail

1

FSA

First Strand Synthesis Act D Mix

1

SMM

Second Strand Marking Master Mix

Box 2, Set A, Store at -25°C to -15°C

Quantity

Reagent

Description

1

AR002

RNA Adapter Index 2

1

AR004

RNA Adapter Index 4

1

AR005

RNA Adapter Index 5

1

AR006

RNA Adapter Index 6

1

AR007

RNA Adapter Index 7

1

AR012

RNA Adapter Index 12

1

AR013

RNA Adapter Index 13

1

AR014

RNA Adapter Index 14

1

AR015

RNA Adapter Index 15

1

AR016

RNA Adapter Index 16

1

AR018

RNA Adapter Index 18

1

AR019

RNA Adapter Index 19

1

ATL2

A-Tailing Mix 2

1

RSB

Resuspension Buffer

1

EPH

Elute, Prime, Fragment High Mix

1

LIG

Ligation Mix

1

ATL

A-Tailing Mix

1

STL

Stop Ligation Buffer

Box 2, Set B, Store at -25°C to -15°C

Quantity

Reagent

Description

1

AR001

RNA Adapter Index 1

1

AR003

RNA Adapter Index 3

1

AR008

RNA Adapter Index 8

1

AR009

RNA Adapter Index 9

1

AR010

RNA Adapter Index 10

1

AR011

RNA Adapter Index 11

1

AR020

RNA Adapter Index 20

1

AR021

RNA Adapter Index 21

1

AR022

RNA Adapter Index 22

1

AR023

RNA Adapter Index 23

1

AR025

RNA Adapter Index 25

1

AR027

RNA Adapter Index 27

1

RSB

Resuspension Buffer

1

EPH

Elute, Prime, Fragment High Mix

1

LIG

Ligation Mix

1

ATL

A-Tailing Mix

1

STL

Stop Ligation Buffer

Box 3, Store at 2°C to 8°C

Quantity

Reagent

Description

1

ET2

Elute Target Buffer 2

3

SMB

Streptavidin Magnetic Beads

Box 4, Store at -25°C to -15°C

Quantity

Reagent

Description

3

EEW

Enhanced Enrichment Wash Buffer

1

CT3

Capture Target Buffer 3

2

EE1

Enrichment Elution Buffer 1

1

EPM

Enhanced PCR Mix

1

HP3

2N NaOH

1

PPC

PCR Primer Cocktail

Box 5, Oligos, Store at -25°C to -15°C

Quantity

Reagent

Description

1

RFO

 

RNA Fusion Oligos