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TruSight Oncology 500 ctDNA Contents & Storage

TruSight Oncology 500 ctDNA Kit (20039252)

Box 1- Library Prep (Pre-Amp), Store at -25°C to -15°C
Quantity Reagent Description
2 UMI UMI Adapters v1
Box 2 - Library Prep (Pre-Amp), Store at -25°C to -15°C
Quantity Reagent Description
2 ALB1 Adapter Ligation Buffer 1
2 EPM Enhanced PCR Mix
2 ERA1-A End Repair A-tailing Enzyme Mix 1
2 ERA1-B End Repair A-tailing Buffer 1
2 LIG3 DNA Ligase 3
2 STL Stop Ligation Buffer
Box 3 - Library Prep (Pre-Amp), See Storage Temperatures in Table
Quantity Reagent Description Storage
1 RSB Resuspension Buffer 2°C to 8°C or -25°C to -15°C
2 SPB Sample Purification Beads 2°C to 8°C
1 TEB TE Buffer 2°C to 8°C
Box 4 - Library Prep - Unique PCR Index Primers (Pre-Amp), Store at -25°C to -15°C
Quantity Reagent Description
1 UP01 Unique Index Primer 01
1 UP02 Unique Index Primer 02
1 UP03 Unique Index Primer 03
1 UP04 Unique Index Primer 04
1 UP05 Unique Index Primer 05
1 UP06 Unique Index Primer 06
1 UP07 Unique Index Primer 07
1 UP08 Unique Index Primer 08
1 UP09 Unique Index Primer 09
1 UP10 Unique Index Primer 10
1 UP11 Unique Index Primer 11
1 UP12 Unique Index Primer 12
1 UP13 Unique Index Primer 13
1 UP14 Unique Index Primer 14
1 UP15 Unique Index Primer 15
1 UP16 Unique Index Primer 16
Box 5 - Enrichment (Post-Amp), See Storage Temperatures in Table
Quantity Reagent Description Storage
2 ET2 Elute Target Buffer 2 2°C to 8°C
2 HP3 2 N NaOH 2°C to 8°C
1 LNB1 Library Normalization Beads 1 2°C to 8°C
2 LNS1 Library Normalization Storage 1 2°C to 8°C
2 LNW1 Library Normalization Wash 1 2°C to 8°C or or -25°C to -15°C
1 RSB Resuspension Buffer 2°C to 8°C or -25°C to -15°C
2 SMB Streptavidin Magnetic Beads 2°C to 8°C
2 SPB Sample Purification Beads 2°C to 8°C
2 TCB1 Target Capture Buffer 1 2°C to 8°C
Box 6 - Enrichment (Post-Amp), Store at -25°C to -15°C
Quantity Reagent Description
3 EE2 Enrichment Elution 2
1 EEW Enhanced Enrichment Wash
2 EPM Enhanced PCR Mix
1 LNA1 Library Normalization Additives 1
2 PPC3 PCR Primer Cocktail 3
2 TCA1 Target Capture Additives 1
Box 7 - Enrichment (Post-Amp), Store at -25°C to -15°C
Quantity Reagent Description
3 OPD2 EOncology DNA Probes Master Pool 2
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