The TruSight Cardio Sequencing Kits contain the following components and is shipped on dry ice. Store the kit components at the specified temperature in designated pre-amplification and post-amplification areas.
TruSight Cardio Sequencing Kit Contents for MiSeq, v2 Chemistry (12 Samples)
Box 1, Store at 2°C to 8°C
Quantity |
Reagent |
Description |
1 |
SPB |
Sample Purification Beads |
1 |
SMB |
Streptavidin Magnetic Beads |
1 |
ET2 |
Elute Target Buffer 2 |
1 |
ST |
Stop Tagment Buffer |
Box 2, Store at -25°C to -15°C
Quantiy |
Reagent |
Description |
1 |
TDE1 |
Tagment DNA Enzyme |
1 |
EE1 |
Enrichment Elution Buffer 1 |
1 |
TD |
Tagment DNA Buffer |
1 |
RSB |
Resuspension Buffer |
1 |
NLM |
Nextera Amplification Mix |
1 |
EHB |
Enrichment Hybridization Buffer |
1 |
EWS |
Enrichment Wash Solution |
1 |
HP3 |
2N NaOH |
1 |
PPC |
PCR Primer Cocktail |
1 |
NEM |
Nextera Enrichment Amplification Mix |
Box 3 Indexes, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
1 |
Index Primer |
E505 |
12 |
Index Primers |
N701 to N712 |
Box 4 Oligos, Store at -25°C to -15°C
Quantity |
Description |
1 |
TruSight Cardio Oligos |
Box 5, PhiX Control v3, Store at -25°C to -15°C
Quantity |
Description |
1 |
PhiX v3 |
MiSeq Reagent Kit v2 Box 1, Store at -25°C to -15°C
Quantity |
Description |
1 |
Hybridization Buffer |
1 |
MiSeq v2 Cartridge, 300 cycles |
MiSeq Reagent Kit v2 Box 2, Store at 2°C to 8°C
Quantity |
Description |
1 |
MiSeq Flow Cell |
1 |
PR2 Bottle |
TruSight Cardio Sequencing Kit Contents for NextSeq, Mid Output (48 Samples)
Box 1 TruSight Rapid Capture Reagents, Store at 2°C to 8°C
Quantity |
Reagent |
Description |
2 |
SPB |
Sample Purification Beads |
2 |
SMB |
Streptavidin Magnetic Beads |
1 |
ET2 |
Elute Target Buffer 2 |
1 |
ST |
Stop Tagment Buffer |
Box 2 TruSight Rapid Capture Reagents, Store at -25°C to -15°C
Quantiy |
Reagent |
Description |
2 |
TDE1 |
Tagment DNA Enzyme |
1 |
EE1 |
Enrichment Elution Buffer 1 |
1 |
TD |
Tagment DNA Buffer |
1 |
RSB |
Resuspension Buffer |
2 |
NLM |
Nextera Amplification Mix |
1 |
EHB |
Enrichment Hybridization Buffer |
1 |
EWS |
Enrichment Wash Solution |
1 |
HP3 |
2N NaOH |
1 |
PPC |
PCR Primer Cocktail |
2 |
NEM |
Nextera Enrichment Amplification Mix |
Box 3 Indexes, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
4 |
Index Primer |
E502 to E506 |
12 |
Index Primers |
N701 to N712 |
Box 4 Oligos, Store at -25°C to -15°C
Quantity |
Description |
4 |
TruSight Cardio Oligos |
Box 5, PhiX Control v3, Store at -25°C to -15°C
Quantity |
Description |
1 |
PhiX v3 |
NextSeq Reagents
Quantity |
Description |
Storage |
1 |
NextSeq 500 Mid Output Reagent Cartridge v2 (300 cycles) |
-25°C to -15°C |
1 |
NextSeq 500 Mid Output Flow Cell |
2°C to 8°C |
1 |
Hybridization Buffer |
Room temperature |