The TruSight Cardio Sequencing Kits contain the following components and is shipped on dry ice. Store the kit components at the specified temperature in designated pre-amplification and post-amplification areas.
TruSight Cardio Sequencing Kit Contents for MiSeq, v2 Chemistry (12 Samples)
Box 1, Store at 2°C to 8°C
| Quantity | Reagent | Description | 
| 1 | SPB | Sample Purification Beads | 
| 1 | SMB | Streptavidin Magnetic Beads | 
| 1 | ET2 | Elute Target Buffer 2 | 
| 1 | ST | Stop Tagment Buffer | 
Box 2, Store at -25°C to -15°C
| Quantiy | Reagent | Description | 
| 1 | TDE1 | Tagment DNA Enzyme | 
| 1 | EE1 | Enrichment Elution Buffer 1 | 
| 1 | TD | Tagment DNA Buffer | 
| 1 | RSB | Resuspension Buffer | 
| 1 | NLM | Nextera Amplification Mix | 
| 1 | EHB | Enrichment Hybridization Buffer | 
| 1 | EWS | Enrichment Wash Solution | 
| 1 | HP3 | 2N NaOH | 
| 1 | PPC | PCR Primer Cocktail | 
| 1 | NEM | Nextera Enrichment Amplification Mix | 
Box 3 Indexes, Store at -25°C to -15°C
| Quantity | Reagent | Description | 
| 1 | Index Primer | E505 | 
| 12 | Index Primers | N701 to N712 | 
Box 4 Oligos, Store at -25°C to -15°C
| Quantity | Description | 
| 1 | TruSight Cardio Oligos | 
Box 5, PhiX Control v3, Store at -25°C to -15°C
| Quantity | Description | 
| 1 | PhiX v3 | 
MiSeq Reagent Kit v2 Box 1, Store at -25°C to -15°C
| Quantity | Description | 
| 1 | Hybridization Buffer | 
| 1 | MiSeq v2 Cartridge, 300 cycles | 
MiSeq Reagent Kit v2 Box 2, Store at 2°C to 8°C
| Quantity | Description | 
| 1 | MiSeq Flow Cell | 
| 1 | PR2 Bottle | 
 
TruSight Cardio Sequencing Kit Contents for NextSeq, Mid Output (48 Samples)
Box 1 TruSight Rapid Capture Reagents, Store at 2°C to 8°C
| Quantity | Reagent | Description | 
| 2 | SPB | Sample Purification Beads | 
| 2 | SMB | Streptavidin Magnetic Beads | 
| 1 | ET2 | Elute Target Buffer 2 | 
| 1 | ST | Stop Tagment Buffer | 
Box 2 TruSight Rapid Capture Reagents, Store at -25°C to -15°C
| Quantiy | Reagent | Description | 
| 2 | TDE1 | Tagment DNA Enzyme | 
| 1 | EE1 | Enrichment Elution Buffer 1 | 
| 1 | TD | Tagment DNA Buffer | 
| 1 | RSB | Resuspension Buffer | 
| 2 | NLM | Nextera Amplification Mix | 
| 1 | EHB | Enrichment Hybridization Buffer | 
| 1 | EWS | Enrichment Wash Solution | 
| 1 | HP3 | 2N NaOH | 
| 1 | PPC | PCR Primer Cocktail | 
| 2 | NEM | Nextera Enrichment Amplification Mix | 
Box 3 Indexes, Store at -25°C to -15°C
| Quantity | Reagent | Description | 
| 4 | Index Primer | E502 to E506 | 
| 12 | Index Primers | N701 to N712 | 
Box 4 Oligos, Store at -25°C to -15°C
| Quantity | Description | 
| 4 | TruSight Cardio Oligos | 
Box 5, PhiX Control v3, Store at -25°C to -15°C
| Quantity | Description | 
| 1 | PhiX v3 | 
NextSeq Reagents
| Quantity | Description | Storage | 
| 1 | NextSeq 500 Mid Output Reagent Cartridge v2 (300 cycles) | -25°C to -15°C | 
| 1 | NextSeq 500 Mid Output Flow Cell | 2°C to 8°C | 
| 1 | Hybridization Buffer | Room temperature |