The TruSeq Rapid Exome Library Prep Kit contains the following components and is shipped on dry ice unless specified otherwise below.
Each kit contains 3 boxes of reagents, 1 box of oligos, and 1 box of index replacement caps.
Quantity per 8 rxn kit |
|
|
|
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1 plex |
3 plex |
6 plex |
9 plex |
12 plex |
Reagent |
Description |
Storage Temperature |
1 |
1 |
2 |
2 |
3 |
SPB |
Sample Purification Beads |
2°C to 8°C |
4 |
4 |
4 |
4 |
4 |
SMB |
Streptavidin Magnetic Beads |
2°C to 8°C |
1 |
1 |
1 |
1 |
1 |
ET2 |
Elute Target Buffer 2 |
2°C to 8°C |
1 |
1 |
1 |
1 |
1 |
EHB2 |
Enrichment Hybridization Buffer 2 |
15°C to 30°C |
1 |
1 |
1 |
1 |
1 |
ST |
Stop Tagment Buffer |
15°C to 30°C |
Quantity per 8 rxn kit |
|
|
||||
1 plex |
3 plex |
6 plex |
9 plex |
12 plex |
Reagent |
Description |
1 |
1 |
1 |
1 |
1 |
RSB |
Resuspension Buffer |
2 |
2 |
2 |
2 |
2 |
EEW |
Enhanced Enrichment Wash Solution |
1 |
1 |
2 |
3 |
1 |
TDE2 |
Tagment DNA Enzyme 2 |
1 |
1 |
1 |
1 |
1 |
EE1 |
Enrichment Elution Buffer 1 |
1 |
1 |
1 |
1 |
1 |
BLR |
Blocker |
1 |
1 |
2 |
3 |
2 |
LAM |
Library Amplification Mix |
1 |
1 |
1 |
1 |
1 |
EHB1 |
Enrichment Hybridization Buffer 1 |
1 |
1 |
1 |
1 |
1 |
HP3 |
2N NaOH |
1 |
1 |
1 |
1 |
1 |
PPC |
PCR Primer Cocktail |
1 |
1 |
1 |
1 |
1 |
EAM |
Enrichment Amplification Mix |
1 |
1 |
1 |
2 |
2 |
TD |
Tagment DNA Buffer |
Quantity per 8 rxn kit |
||||||
1 plex |
3 plex |
6 plex |
9 plex |
12 plex |
Reagent |
Description |
1 |
1 |
2 |
3 |
– |
E502 |
E502 Index Adapter |
– |
– |
– |
– |
2 |
E505 |
E505 Index Adapter |
– |
– |
– |
– |
2 |
E506 |
E506 Index Adapter |
1 |
– |
1 |
1 |
1 |
N701 |
N701 Index Adapter |
– |
1 |
1 |
1 |
1 |
N702 |
N702 Index Adapter |
– |
– |
1 |
1 |
1 |
N703 |
N703 Index Adapter |
– |
1 |
1 |
1 |
1 |
N704 |
N704 Index Adapter |
– |
1 |
1 |
1 |
1 |
N705 |
N705 Index Adapter |
– |
– |
– |
– |
1 |
N706 |
N706 Index Adapter |
– |
– |
1 |
1 |
1 |
N707 |
N707 Index Adapter |
– |
– |
– |
– |
1 |
N708 |
N708 Index Adapter |
– |
– |
– |
– |
1 |
N709 |
N709 Index Adapter |
– |
– |
– |
1 |
1 |
N710 |
N710 Index Adapter |
– |
– |
– |
1 |
1 |
N711 |
N711 Index Adapter |
– |
– |
– |
1 |
1 |
N712 |
N712 Index Adapter |
Quantity |
Reagent |
Description |
4 |
CEX |
Coding Exome Oligos |
Quantity |
Description |
1 |
i7 Index Tube Caps, Orange |
1 |
i5 Index Tube Caps, White |