Library Prep Reagents
Box 1 - DNA Ampicon Assay v2 (16 Samples), Store at -25°C to -15°C
| Quantity |
Reagent |
Description |
| 1 |
ACP3 |
Amplicon Control Oligo Pool 3 |
| 1 |
EDP |
Enhanced DNA Polymerase |
| 1 |
ELB |
Extension-Ligation Buffer |
| 1 |
ELE |
Extension-Ligation Enzyme |
| 1 |
EMM |
Enhanced Master Mix |
Box 1 also contains the HYP barcode label.
Box 2 - DNA Amplicon Assay v2 (16 Samples)
| Quantity |
Reagent |
Description |
Storage |
| 1 |
LNB1 |
Library Normalization Beads |
2°C to 8°C |
| 1 |
OHS2 |
Oligo Hybridization for Sequencing Reagent 2 |
2°C to 8°C |
| 1 |
RS1 |
Resuspension Solution 1 |
15°C to 30°C |
| 1 |
SPB |
Sample Purification Beads |
2°C to 8°C |
| 1 |
SS1 |
Sample Stabilization Solution 1 |
2°C to 8°C |
| 1 |
SW1 |
Stringent Wash 1 |
2°C to 8°C |
Box 3 - DNA Amplicon Assay v2 (16 Samples) (Pre-PCR)
| Quantity |
Reagent |
Description |
Storage |
| 1 |
HT1 |
Hybridization Buffer |
-25°C to -15°C |
| 1 |
LNA1 |
Library Normalization Additives 1 |
-25°C to -15°C |
| 1 |
LNS2 |
Library Normalization Storage Buffer 2 |
15°C to 30°C |
| 2 |
LNW1 |
Library Normalization Wash 1 |
2°C to 8°C |
| 2 |
RSB |
Resuspension Buffer |
15°C to 30°C |
Box 3 also contains the CLP, LNP, SGP, PAL, and DAL barcode labels.
Index Adapters
Nextera XT Index Kit v2 Set A (96 Indexes, 192 Samples), Store at -25°C to -15°C
| Quantity |
Reagent |
| 8 tubes |
Index Adapters S502, S503, S505–S508, S510, and S511 |
| 12 tubes |
Index Adapters N701–N707, N710–N712, N714, and N715 |
Nextera XT Index Kit v2 Set B (96 Indexes, 192 Samples), Store at -25°C to -15°C
| Quantity |
Reagent |
| 8 tubes |
Index Adapters S502, S503, S505–S508, S510, and S511 |
| 12 tubes |
Index Adapters N716, N718–N724, and N726–N729 |
Nextera XT Index Kit v2 Set C (96 Indexes, 192 Samples), Store at -25°C to -15°C
| Quantity |
Reagent |
| 8 tubes |
Index Adapters SS513, S515–S518, and S520–S522 |
| 12 tubes |
Index Adapters N701–N707, N710–N712, N714, and N715 |
Nextera XT Index Kit v2 Set D (96 Indexes, 192 Samples), Store at -25°C to -15°C
| Quantity |
Reagent |
| 8 tubes |
Index Adapters S513, S515–S518, and S520–S522 |
| 12 tubes |
Index Adapters N716, N718–N724, and N726–N729 |
Sequencing Reagents
MiniSeq Reagents
| Quantity |
Reagent |
Storage |
| 1 |
MiniSeq Reagent Cartridge (150 cycles) |
-25°C to -15°C |
| 1 |
MiniSeq Flow Cell |
2°C to 8°C |
| 1 |
Hybridization Buffer (HT1) |
-25°C to -15°C |
NextSeq Reagents
| Quantity |
Reagent |
Storage |
| 1 |
NextSeq 500 High Output Reagent Cartridge v2 (150 cycles) |
-25°C to -15°C |
| 1 |
NextSeq 500 High Output Flow Cell v2 |
2°C to 8°C |
| 1 |
NextSeq 500/550 Buffer Cartridge |
-25°C to -15°C |
| 1 |
NextSeq Accessory Box v2 |
-25°C to -15°C |
MiSeq Reagent Kit v3 - Box 1, Store at -25°C to -15°C
| Quantity |
Reagent |
| 1 |
MiSeq v3 Reagent Tray (150 cycles, PE) |
| 1 |
Hybridization Buffer (HT1) |
MiSeq Reagent Kit v3 - Box 2, Store at 2°C to 8°C
| Quantity |
Reagent |
| 1 |
PE MiSeq Flow Cell |
| 1 |
Incorporation Buffer (PR2) |