| Quantity | Reagent | Description | 
 | 
| 1 | RSB | Resuspension Buffer | |
| 1 | ERP 2 or ERP 3 | End Repair Mix | |
| 1 | ATL or ATL 2 | A-Tailing Mix | |
| 1 | LIG 2 | Ligation Mix 2 | |
| 1 | CTE | End Repair Control | |
| 1 | CTA | A-Tailing Control | |
| 1 | CTL | Ligation Control | |
| 1 | STL | Stop Ligation Buffer | 
| Quantity | Reagent | Description | 
 | 
| 1 | SPB | Sample Purification Beads | 
| Quantity | Reagent | Description | 
 | 
| 2 | RSB | Resuspension Buffer | |
| 2 | ERP 2 or ERP 3 | End Repair Mix | |
| 2 | ATL or ATL 2 | A-Tailing Mix | |
| 2 | LIG 2 | Ligation Mix 2 | |
| 2 | CTE | End Repair Control | |
| 2 | CTA | A-Tailing Control | |
| 2 | CTL | Ligation Control | |
| 2 | STL | Stop Ligation Buffer | 
| Quantity | Reagent | Description | 
 | 
| 4 | SPB | Sample Purification Beads |