Contents & Storage

The Nextera Mate Pair Sample Preparation Kit Box 1 and Box 2 contain the reagents required up to the End Repair step. The TruSeq Sample Prep Kit boxes and the Nextera Mate Pair Sample Preparation Kit - Wash Solutions Boxprovide the reagents required for the End Repair step onwards.

Nextera Mate Pair Sample Preparation Kit - Box 1

Reagent

Storage

Description

MTP

-15° to -25°C

Mate Pair Tagment Enzyme

NPT

-15° to -25°C

dNTPs

SDP

-15° to -25°C

Strand Displacement Polymerase

PS1

-15° to -25°C

Exonuclease

TB1

-15° to -25°C

Tagment Buffer Mate Pair

NT

Room temperature

Neutralize Tagment Buffer

CB

-15° to -25°C

Circularization Buffer 10X

SDB

-15° to -25°C

10X Strand Disp Buffer

CL

-15° to -25°C

Circularization Ligase

STL*

-15° to -25°C

Stop Ligation Buffer*

*Two Tubes of Stop Ligation Buffer are provided in the Nextera Mate Pair Sample Preparation Kit: one tube in the Nextera Mate Pair Sample Preparation Kit – Box 1, and another tube in the TruSeq DNA LT Sample Prep – Set A box.

Nextera Mate Pair Sample Preparation Kit – Box 2 Wash Solutions

RSB

Room temperature

Resuspension Buffer*

BBB

Room temperature

Bead Bind Buffer

BWB

Room temperature

Bead Wash Buffer

*Two aliquots of Resuspension Buffer are provided in the Nextera Mate Pair Sample Preparation Kit: One larger volume bottle in the Nextera Mate Pair Sample Preparation Kit – Box 2, and a second smaller volume tube in the TruSeq DNA LT Sample Prep – Set A box.

TruSeq DNA LT Sample Prep Kit - Set A Box

Reagent

Storage

Description

RSB

-15° to -25°C

Resuspension Buffer

ERP

-15° to -25°C

End Repair Mix

ATL

-15° to -25°C

A-Tailing Mix

LIG

-15° to -25°C

Ligation Mix

CTE

-15° to -25°C

End Repair Control

CTA

-15° to -25°C

A-Tailing Control

CTL

-15° to -25°C

Ligation Control

STL

-15° to -25°C

Stop Ligation Buffer

AD002

-15° to -25°C

DNA Adapter Index 2

AD004

-15° to -25°C

DNA Adapter Index 4

AD005

-15° to -25°C

DNA Adapter Index 5

AD006

-15° to -25°C

DNA Adapter Index 6

AD007

-15° to -25°C

DNA Adapter Index 7

AD012

-15° to -25°C

DNA Adapter Index 12

AD013

-15° to -25°C

DNA Adapter Index 13

AD014

-15° to -25°C

DNA Adapter Index 14

AD015

-15° to -25°C

DNA Adapter Index 15

AD016

-15° to -25°C

DNA Adapter Index 16

AD018

-15° to -25°C

DNA Adapter Index 18

AD019

-15° to -25°C

DNA Adapter Index 19

The control reagents supplied in the TruSeq LT Sample Prep Kit cannot be used when preparing Nextera Mate Pair Sample Preparation Libraries. However, these controls can still be used with the TruSeq DNA LT Sample Preps kit if performing Standard TruSeq DNA Sample Preparation.

TruSeq DNA LT Sample Prep Kit - PCR Box

Reagent

Storage

Description

EPM

-15° to -25°C

Enhanced PCR Mix

PPC

-15° to -25°C

PCR Primer Cocktail