The Nextera Mate Pair Sample Preparation Kit Box 1 and Box 2 contain the reagents required up to the End Repair step. The TruSeq Sample Prep Kit boxes and the Nextera Mate Pair Sample Preparation Kit - Wash Solutions Boxprovide the reagents required for the End Repair step onwards.
Reagent |
Storage |
Description |
MTP |
-15° to -25°C |
Mate Pair Tagment Enzyme |
NPT |
-15° to -25°C |
dNTPs |
SDP |
-15° to -25°C |
Strand Displacement Polymerase |
PS1 |
-15° to -25°C |
Exonuclease |
TB1 |
-15° to -25°C |
Tagment Buffer Mate Pair |
NT |
Room temperature |
Neutralize Tagment Buffer |
CB |
-15° to -25°C |
Circularization Buffer 10X |
SDB |
-15° to -25°C |
10X Strand Disp Buffer |
CL |
-15° to -25°C |
Circularization Ligase |
STL* |
-15° to -25°C |
Stop Ligation Buffer* |
*Two Tubes of Stop Ligation Buffer are provided in the Nextera Mate Pair Sample Preparation Kit: one tube in the Nextera Mate Pair Sample Preparation Kit – Box 1, and another tube in the TruSeq DNA LT Sample Prep – Set A box.
RSB |
Room temperature |
Resuspension Buffer* |
BBB |
Room temperature |
Bead Bind Buffer |
BWB |
Room temperature |
Bead Wash Buffer |
*Two aliquots of Resuspension Buffer are provided in the Nextera Mate Pair Sample Preparation Kit: One larger volume bottle in the Nextera Mate Pair Sample Preparation Kit – Box 2, and a second smaller volume tube in the TruSeq DNA LT Sample Prep – Set A box.
Reagent |
Storage |
Description |
RSB |
-15° to -25°C |
Resuspension Buffer |
ERP |
-15° to -25°C |
End Repair Mix |
ATL |
-15° to -25°C |
A-Tailing Mix |
LIG |
-15° to -25°C |
Ligation Mix |
CTE |
-15° to -25°C |
End Repair Control |
CTA |
-15° to -25°C |
A-Tailing Control |
CTL |
-15° to -25°C |
Ligation Control |
STL |
-15° to -25°C |
Stop Ligation Buffer |
AD002 |
-15° to -25°C |
DNA Adapter Index 2 |
AD004 |
-15° to -25°C |
DNA Adapter Index 4 |
AD005 |
-15° to -25°C |
DNA Adapter Index 5 |
AD006 |
-15° to -25°C |
DNA Adapter Index 6 |
AD007 |
-15° to -25°C |
DNA Adapter Index 7 |
AD012 |
-15° to -25°C |
DNA Adapter Index 12 |
AD013 |
-15° to -25°C |
DNA Adapter Index 13 |
AD014 |
-15° to -25°C |
DNA Adapter Index 14 |
AD015 |
-15° to -25°C |
DNA Adapter Index 15 |
AD016 |
-15° to -25°C |
DNA Adapter Index 16 |
AD018 |
-15° to -25°C |
DNA Adapter Index 18 |
AD019 |
-15° to -25°C |
DNA Adapter Index 19 |
The control reagents supplied in the TruSeq LT Sample Prep Kit cannot be used when preparing Nextera Mate Pair Sample Preparation Libraries. However, these controls can still be used with the TruSeq DNA LT Sample Preps kit if performing Standard TruSeq DNA Sample Preparation.
Reagent |
Storage |
Description |
EPM |
-15° to -25°C |
Enhanced PCR Mix |
PPC |
-15° to -25°C |
PCR Primer Cocktail |