Before preparing libraries, create a sample sheet to record the index adapter combinations and other information about your samples. Sample sheet options and requirements depend on the instrument. For information, see the instrument support pages.
The Nextera XT Index Kit v2 Sets A–D are compatible with the Nextera DNA XT Library Prep Kit and bead-based normalization. For standard normalization, use the IDT for Illumina Nextera DNA UD Indexes.
The Nextera XT Index Kit v2 replaces the Nextera XT Indexes (96 indexes, 384 samples) (catalog # FC-131-1002). Nextera XT Indexes (24 indexes, 96 samples) (catalog # FC-131-1001) are still available.