IDT for Illumina TruSeq UD Indexes are unique dual (UD) indexes with distinct, unrelated adapters for both index reads. They support DNA and RNA library preps and various plexities. The index adapters sequences are eight bases long.
Before preparing libraries, create a sample sheet to record the index adapter combinations and other information about your samples. Sample sheet options and requirements depend on the instrument. For information, see the instrument support pages.
To use IDT for Illumina TruSeq UD Indexes with Local Run Manager, add a new library kit and upload the IDT for Illumina TruSeq UD Indexes Sample Sheet Template for Local Run Manager. For instructions, see the Local Run Manager Software Guide.
Use the IDT for Illumina TruSeq UD Indexes Sample Sheet Templates for IEM to record sequences for IDT for Illumina TruSeq UD Indexes.
IDT for Illumina TruSeq UD Indexes are compatible with the following library prep kits.
The following instruments can sequence libraries ligated with IDT for Illumina TruSeq UD Indexes. For library prep kit and instrument compatibility, see the support pages for your kit or instrument.