HiSeq 2500 Documentation

Instructions for preparing PhiX and denaturing and diluting libraries for sequencing on the HiSeq 2500, HiSeq 1500, or HiSeq 2000 System.

Instructions for sequencing with custom primers on any HiSeq system (except the HiSeq X System).

The safety and compliance guide describes safety considerations and lists product compliance markings for the HiSeq 2500 system.

Primer rehybridization instructions using HiSeq v4 or TruSeq v3 chemistry.

Instructions for performing primer rehybridization on the HiSeq using the HiSeq Rapid Rehybridization Kit or the TruSeq Rapid Rehybridization Kit.

Instructions for using the TruSeq v2 cBot Multi-Primer Re-Hybridization Kit for primer rehybridization on a TruSeq v3 Flow Cell.

Strategies for optimizing cluster density and preventing and diagnosing clustering issues on Illumina flow cells.

The sequencing process for single- and dual-indexed runs on Illumina instruments.

Guidelines for managing the security of the control computer on your sequencing system. Use these guidelines to configure your system and ensure a more secure operating environment.

It is strongly recommended that you review this documentation to keep your instrument secure.