Quantity |
Reagent |
Description |
1 |
cBot Reagent Plate |
PE Kit—cBot reagent plate, PE SR Kit—cBot reagent plate, SR |
1 |
RSB |
Resuspension Buffer |
1 |
EPX1 |
Enhanced Pattern Cluster Mix 1 |
1 |
EPX2 |
Enhanced Pattern Cluster Mix 2 |
1 |
EPX3 |
Enhanced Pattern Cluster Mix 3 |
Quantity |
Reagent |
Description |
1 |
HRM |
HT Resynthesis Mix |
1 |
HLM2 |
HT Linearization Mix 2 |
1 |
HAM |
HT Amplification Mix |
1 |
HPM |
HT Amp Premix |
1 |
HDR |
HT Denaturation Mix (contains formamide) |
1 |
HP11 |
Primer Mix – Read 2 |
1 |
HP14 |
Indexing Primer Mix |
Quantity |
Reagent |
Description |
1 |
HRM |
HT Resynthesis Mix |
1 |
HDR |
HT Denaturation Mix |
1 |
HP12 |
Index 1 Primer Mix |
Quantity |
Accessory |
Purpose |
1 |
Flow cell gaskets (package of 4) |
Replace the flow cell gaskets between runs. |
1 |
Flow cell storage tube with storage buffer, 50 ml |
Store the flow cell between clustering and sequencing. |
1 |
Funnel bottle caps (package of 8) |
Replace the caps on SBS reagent bottles before loading reagents. |
The HiSeq 3000/4000 Cluster Kit also includes the following:
Kit Component |
Storage |
Description |
PW1 |
15°C to 30°C | Wash Buffer |
HB1 | 15°C to 30°C | High Throughput SBS Buffer 1 |
HB2 | 15°C to 30°C | High Throughput SBS Buffer 2 |
Kit Component |
Storage |
Description |
HCM |
-25°C to -15°C |
High Throughput Cleavage Mix |
HIM |
-25°C to -15°C | High Throughput Incorporation Mix |
HSM | -25°C to -15°C | High Throughput Scan Mix |
HiSeq SBS kits contain a sufficient volume of reagents for performing dual-indexed runs:
The following table lists the number of cycles performed for dual-indexed paired-end runs of various lengths. Run cycles comprise several sequencing cycles based on run length and 2 index reads of 8 cycles each. For dual-indexed single-read runs, 7 chemistry-only cycles are calculated in the total number of cycles.
Run Length |
Run Length |
Run Cycles |
Total Cycles |
Dual-Indexed Paired End |
2 x 150 |
151+8+8+151 |
318 |
2 x 100 |
101+8+8+101 |
218 |
|
2 x 75 |
76+8+8+76 |
168 |
|
2 x 50 |
51+8+8+51 |
118 |
|
2 x 35 |
36+8+8+36 |
88 |
|
Dual-Indexed Single-Read |
1 x 150 |
151+8+(7)+8 |
174 |
1 x 100 |
101+8+(7)+8 |
124 |
|
1 x 75 |
76+8+(7)+8 |
99 |
|
1 x 50 |
51+8+(7)+8 |
74 |
|
1 x 35 |
36+8+(7)+8 |
59 |
See Safety Data Sheets.