Infinium HumanMethylation450K BeadChip Contents & Storage

STM (Make sure that all STM tubes indicate the
same stain temperature on the label)

2 tubes

-15° to -25°C

XC4

310 ml

-15° to -25°C

XC2

2 tubes

-15° to -25°C

ATM

2 tubes

-15° to -25°C

RA1

If you are performing an FFPE protocol, use 30 µl per sample well;
otherwise, use 46 µl per sample well

-15° to -25°C

FMS

If you are performing an FFPE protocol, use 1 tube per 96 samples;
otherwise, use 2 tubes per 96 samples

-15° to -25°C

RPM

2 tubes
(per 96 samples)

-15° to -25°C

MSM

2 tubes
(per 96 samples)

-15° to -25°C

XC1

2 tubes

-15° to -25°C

RA1

10 ml

-15° to -25°C

TEM

2 tubes

-15° to -25°C

PM1

2 tubes (per 96 samples)

2° to 8°C

PB2

2 tubes

Room temperature

Te-Flow Flow-Through Chambers (with Black Frames,
Spacers,Glass Back Plates, and Clamps)

1 (per BeadChip)

Room temperature

Multi-Sample BeadChip Alignment Fixture

1 (per 8 BeadChips)

Room temperature

Hyb Chamber inserts

8

Room temperature

PB1

550 ml (up to 8 BeadChips)

Room temperature

Wash Dish

2 (up to 8 BeadChips)

Room temperature

Wash Rack

1 (up to 8 BeadChips)

Room temperature

Alconox Powder Detergent

as needed

Room temperature

EtOH

as needed

Room temperature

MA1

2 tubes
(per 96 samples)

Room temperature

XC3

75 ml

Room temperature

Hyb Chambers

2

Room temperature

Hyb Chamber gaskets

2

Room temperature

PB1

310 ml

Room temperature

BeadChips

8

2° to 8°C

95% formamide/1 mM EDTA

15 ml

-15° to -25°C

BCD plate with Bisulfite-converted DNA samples

1 plate

-15° to -25°C

Lambda DNA

See Instructions

2° to 8°C

0.1N NaOH

15 ml (per 96 samples)

2° to 8°C

PicoGreen dsDNA quantitation reagent

See Instructions

2° to 8°C

Zymo EZ DNA Methylation kit (includes bisulfite-conversion
reagent, dilution buffer, desulphonation buffer, elution buffer)

1 kit per 50 samples

1 kit per 200 samples

or

1 kit per 2 plates

Room temperature

Fluotrac 200 96-well flat-bottom plate

1 per Std DNA plate

1 per Sample DNA plate

Room temperature

Genomic DNA (for HD Methylation)

500 ng for each bisulfite conversion reaction for manual process

1000 ng for each bisulfite conversion reaction for the Automated process

Room temperature

EtOH

500 ml

Room temperature

96-well 0.2 ml skirted microplate

1–3 plates

Room temperature

1X TE

See Instructions

Room temperature

96-well 0.8 ml microtiter plate (MIDI)

1 plate

Room temperature

100% 2-propanol

40 ml (per 96 samples)

Room temperature

96-well 0.65 ml microtiter plate

1 per 96 samples

Room temperature