Infinium Mouse Methylation BeadChip Contents & Storage

95% formamide/1 mM EDTA

15 ml

-15° to -25°C

BCD plate with Bisulfite-converted DNA samples

1 plate

-15° to -25°C

Lambda DNA

See Instructions

2° to 8°C

0.1N NaOH

15 ml (per 96 samples)

2° to 8°C

PicoGreen dsDNA quantitation reagent

See Instructions

2° to 8°C

Zymo EZ DNA Methylation kit (includes bisulfite-conversion reagent,
dilution buffer, desulphonation buffer, elution buffer)

1 kit per 50 samples

1 kit per 200 samples

or

1 kit per 2 plates

Room temperature

Fluotrac 200 96-well flat-bottom plate

1 per Std DNA plate

1 per Sample DNA plate

Room temperature

Genomic DNA (for HD Methylation)

500 ng for each bisulfite conversion reaction for manual process

1000 ng for each bisulfite conversion reaction for the Automated process

Room temperature

EtOH

500 ml

Room temperature

96-well 0.2 ml skirted microplate

1–3 plates

Room temperature

1X TE

See Instructions

Room temperature

96-well 0.8 ml microtiter plate (MIDI)

1 plate

Room temperature

100% 2-propanol

40 ml (per 96 samples)

Room temperature

96-well 0.65 ml microtiter plate

1 per 96 samples

Room temperature