Installing the GRCh38Decoy Reference Genome Nirvana Annotation Database

1. Ensure that the DNA Amplicon Module version installed is version 1.1.0 or greater. This Nirvana Annotation Database is only 
   compatible with version 1.1.0 or greater.

2. Download the "Nirvana_GRCh38Decoy.zip" file for the GRCh38Decoy Reference Genome from the Local Run Manager Software Downloads Page.

3. Right click on the "Nirvana_GRCh38Decoy.zip" file and select "Extract All..."

4. Once the extraction is complete, open the folder Nirvana_GRCh38Decoy and move the Nirvana folder to: 
	 <Local Run Manager Reference Genome Repository>\Homo_sapiens\NCBI\GRCh38Decoy\Annotation

5. Once the move has completed, there should now be a 
   <Local Run Manager Reference Genome Repository>\Homo_sapiens\NCBI\GRCh38Decoy\Annotation\Nirvana folder 
   
   Within the Nirvana folder, the following folders should be present 
   <Local Run Manager Reference Genome Repository>\Homo_sapiens\NCBI\GRCh38Decoy\Annotation\Nirvana\Cache\26
   <Local Run Manager Reference Genome Repository>\Homo_sapiens\NCBI\GRCh38Decoy\Annotation\Nirvana\SupplementaryDatabase\44
   <Local Run Manager Reference Genome Repository>\Homo_sapiens\NCBI\GRCh38Decoy\Annotation\Nirvana\Reference\5

   (default reference genome repository: "C:\Illumina\Genomes\").
   (default reference genome repository MiSeq 2.x: "C:\Illumina\MiSeq Reporter\Genomes")
   
6. Delete the "Nirvana_GRCh38Decoy.zip" file to save disk space.